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SUMO-tag vs His-tag vs GST-tag vs MBP-tag: Which Is Best for Protein Expression?Recombinant protein expression is a cornerstone of modern biochemistry, structural biology, and biopharmaceutical research. However, researchers frequently encounter challenges such as low yield, aggregation, inclusion body formation, and proteolytic degradation—particularly when expressing proteins in Escherichia coli. In many cases, the difference between a failed experiment and a...0 Commentarios 0 Acciones 445 Views 0 Vista previa